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STEMCELL Technologies Inc stemspan tm cc100
Stemspan Tm Cc100, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+tm+cc100/stemspan+cc100/pmc12266127-309-23-26
Average 90 stars, based on 1 article reviews
stemspan tm cc100 - by Bioz Stars, 2026-09
90/100 stars

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Cell Culture:

Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
Article Snippet: Next, the cells were stained against the macrophage marker CD11b (1:200 of CD11b-APC, BD Pharmingen, catalog no. 550019) and CD14 (1:200 of CD14-APC, BD Pharmigen, catalog no. 561383) and resuspended in PBS containing DAPI as a viability marker. .. Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions. ..

Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
Article Snippet: .. Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690). ..

shRNA:

Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
Article Snippet: Next, the cells were stained against the macrophage marker CD11b (1:200 of CD11b-APC, BD Pharmingen, catalog no. 550019) and CD14 (1:200 of CD14-APC, BD Pharmigen, catalog no. 561383) and resuspended in PBS containing DAPI as a viability marker. .. Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions. ..

other:

Article Title: The Laminin Receptors Basal Cell Adhesion Molecule/Lutheran and Integrin α7β1 on Human Hematopoietic Stem Cells
Article Snippet: 1 × 10 4 MACS-purified CD34 + HSPC were incubated in duplicates with soluble laminin isoforms (LM-211, LM-411, LM-511 and LM-521), with mouse anti-human BCAM monoclonal antibody or with PBS in 100 μl of serum free expansion medium (SFEM) containing 100 ng/ml stem cell factor, 100 ng/ml Flt-3 ligand, 20 ng/ml interleukin-3 and 20 ng/ml interleukin-6 (StemSpan TM CC100, Stem Cell Technologies, Vancouver, BC, Canada) for 1–3 days in a flat bottom 96 well plate in a humidified atmosphere with 5% CO 2 .

Generated:

Article Title: Replication stress responses in human lymphocytes change sex-specifically during aging
Article Snippet: .. Primary HSPC cultures were generated by CD34+ enrichment (CD34 MicroBead kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and cultivated for 72 h at a cell density of 0.75 × 10 6 cells/ml in StemSpan TM SFEM supplemented with StemSpan TM CC100 (STEMCELL Technologies, Vancouver, BC, Canada) as described previously [ ]. ..



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